Determination method of serum albumin using a Bromocresol Green solution and a Bromocresol Purple solution containing no detergent as a color reagent.
نویسندگان
چکیده
منابع مشابه
Bromocresol purple, a non-specific colour reagent for the determination of serum albumin.
The determination of serum albumin with the dye reagent bromocresol purple was investigated. We found that bromocresol purple is not a specific reagent for albumin, but that serum proteins in the alpha-, beta-, and gamma-globulin fractions also react with this dye. Similar results were obtained for bromocresol green. Furthermore there is a great difference in absorbance between human and bovine...
متن کاملMeasurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods.
BACKGROUND/AIMS The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), ...
متن کاملDetermination of caprine serum albumin in milk using bromocresol green dye
The objective of this study was to develop and validate a colorimetric assay to measure caprine serum albumin (CSA) concentrations in milk. The principle of the say was based on the binding of albumin to bromocresol green (BCG) dye at pH 4.0. The absorption of the dye-albumin complex was determined spectrophotometrically at 640 nm. A linear relationship existed between CSA concentrations (0.01 ...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 2003
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.52.269